(1) The WEP is resolutely neither leftwing nor rightwing but non-partisan.(20) N-ethyl piperidinyl diaminodithiol (NEP-DADT), complexed with 99mTc has been developed as an agent for the measurement of brain blood flow using SPECT.(19) To determine the distribution of NEP, a possible regulatory enzyme for the neuropeptide-induced leukocyte activation, among human leukocytes, we investigated the enzymatic activity of NEP in each cell type of human peripheral blood leukocytes.(18) We concluded that, although covalent bonds appear to be cleaved in NEP by papain, its activity and structure are sustained by S-S bridges.(17) Using NEP-expressing MDCK cells and episcopic fluorescence microscopy, a specific labeling was obtained with 100 nM FTI which was completely displaced by 10 microM HACBOGly, a specific and potent inhibitor of NEP.(16) This binding was displaced in a dose-dependent manner by NEP inhibitors.(15) Also examined were the effects of inhibitors of neutral endopeptidase (NEP) and angiotensin-converting enzyme (ACE).(14) There were no significant differences in apparent volume of distribution, elimination half-life, and elimination clearance (CIe) for nEp and rEp in intact dogs.(13) The activity of NEP, measured with an enzymatic fluorimetric method employing N-dansyl-D-alanyl-glycyl-L-4-nitrophenylalanyl-glycine as a synthetic substrate, was 18 times and eight times higher in the outer stripe of the medulla and inner cortex than in the outer cortex (OC).
(10) On the other hand, transient or enhanced expression of NEP is observed during the development of several organs such as the sensory organs, the heart and the major blood vessels, the intestine, the bones and the genital tubercle.NEP attacked MCH at three sites of the molecule with an apparent affinity of about 12 microM and a kcat. (9) The present work examined the susceptibility of these novel peptides to hydrolysis by various purified exo- and endo-peptidases including endopeptidases 24.11 (NEP), 24.15, 24.16, angiotensin-converting enzyme, leucine aminopeptidase and carboxypeptidase A.(8) The effects of neutral endopeptidase inhibition (NEP-I) were studied in 6 conscious sheep with heart failure (HF) induced by rapid ventricular pacing for 7 days.
(7) We gave phosphoramidon to inhibit NEP and enalapril maleate or captopril to inhibit ACE.(6) By cleaving and thus inactivating tachykinins released from sensory nerves, NEP limits the actions of these peptides.(5) These results suggest that degradation of NKA and NKB by NEP but not by ACE is an important determinant of the bronchoconstriction induced by these peptides.(4) The effects of the neutral metalloendopeptidase (NEP) inhibitor, thiorphan, and the angiotensin converting enzyme (ACE) inhibitor, captopril, on airway responses to SP5-11 were examined in order to test the hypothesis that differences in degradation of SP and SP5-11 contribute to the difference in airway responsiveness to the two peptides.(3) The nuclear masses are known as (1) the area lateralis hypothalami (ALH), (2) the nucleus entopeduncularis (nEp), (3) the nucleus semilunaris accessorius (nSA), (4) the nucleus ventromedialis hypothalami (nVmH), and (5) the posterolateral portion of the zona incerta (ZI).(2) These data indicate that mammalian tachykinins induce the release of NCA from BBEC and that NEP modulates these effects.
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